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Gene expression profile at the single cell level of CD45+ CD11b+ cells isolated from the brain and bone marrow of mice either untreated or conditioned and transplanted with mouse hematopoietic stem and progenitor cells (HSPCs) expanded in culture.

GSE261246 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/13 Platform GPL24247
Summary
We used single-cell RNA sequencing (scRNA-seq) to analyze the diversity of HSPC-derived CD45+CD11b+ microglia-like cells (MGLCs) engrafted in the brain of recipient mice that were conditioned using Busulfan and PLX3397 and transplanted intravenous with Lineage negative KIT+ SCA1+ mouse HSPCs. The HSPCs were isolated from adult C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ homozygous mice. We compared the gene expression of MGLCs to that of developmentally-derived CD45+ CD11b+ microglia/myeloid cells isolated from the brain of recipient mice (host microglia) and untreated mice (naive microglia). We also compared the gene expression of MGLCs to that of transplant-derived CD45+ CD11b+ cells engrafted in the bone marrow (abbreviated as BM-CD11b+)
Published in
CNS-wide repopulation by hematopoietic-derived microglia-like cells corrects progranulin deficiency in mice
Colella P, Sayana R, Suarez-Nieto MV et al. · Nature communications 2024 · PMID 38969669 · doi:10.1038/s41467-024-49908-4
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Also filed as BioProject PRJNA1086156 and SRA study SRP494351. Searching any of these in the dataset finder brings you back here.

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