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Semaphorin 3E-Plexin-D1 pathway regulates ovulation, granulosa cell luteinization, and ovarian angiogenesis in mice

GSE262209 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/08/01 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × mouse ovarian granulosa cells, IgG injected, 8 h post-hCG, vs 3 × mouse ovarian granulosa cells, Sema3E injected, 8 h post-hCG, vs 3 × mouse ovarian stromal tissues, IgG injected, 8 h post-hCG, vs 3 × mouse ovarian stromal tissues, Sema3E injected, 8 h post-hCG,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Semaphorin 3E-Plexin-D1 Pathway Downstream of the Luteinizing Hormone Surge Regulates Ovulation, Granulosa Cell Luteinization, and Ovarian Angiogenesis in MiceAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
Summary (written by the submitters)
Ovulation is induced by the luteinizing hormone surge and accompanied by granulosa cell luteinization and ovarian angiogenesis. Sema3E-Plexin-D1 pathway regulates angiogenesis in other tissues, but its role in the ovary was unknown. We report herein that the expression of Sema3E and its receptor, Plexin-D1, is dynamically regulated in the mouse ovary downstream of the LH surge.
Read the full summary ↓
This regulation is mediated by CCAAT/enhancer-binding proteins α and β through modulating chromatin accessibility. Intraovarian injection of recombinant Sema3E results in reduced ovulation, impaired corpus luteum formation, and aberrant ovarian angiogenesis. These in vivo physiological abnormalities are consistent with altered expression of genes regulating these processes, and with data from in vitro cultured granulosa cells and/or ovarian stromal tissues treated with Sema3E or Plexin-D1 neutralizing antibody. Our findings shed light on the important role of Sema3E-Plexin-D1 pathway in the ovary, pinpointing it as a potential therapeutic target for fertility and infertility management.
Published in
Semaphorin 3E-Plexin-D1 Pathway Downstream of the Luteinizing Hormone Surge Regulates Ovulation, Granulosa Cell Luteinization, and Ovarian Angiogenesis in Mice
Zhang H, Dhillon J, Soloway PD et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 · PMID 40391781 · doi:10.1002/advs.202417163
This dataset
Open GSE262209 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE262209_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1087375 and SRA study SRP496417. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40391781) ↗
Study design
3 × mouse ovarian granulosa cells, IgG injected, 8 h post-hCG, vs 3 × mouse ovarian granulosa cells, Sema3E injected, 8 h post-hCG, vs 3 × mouse ovarian stromal tissues, IgG injected, 8 h post-hCG, vs 3 × mouse ovarian stromal tissues, Sema3E injected, 8 h post-hCG,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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