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An Lgr5-independent developmental lineage is involved in mouse intestinal regeneration

GSE262572 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/03/19 Platform GPL24247
Summary
Collagenase/dispase treatment of intestinal tissue from adult mice generates cells growing in matrigel as immortal cystic spheroids in addition to differentiated organoids. Contrary to classical EDTA-derived organoids, these spheroids display poor intestinal differentiation and are independent of Rspondin/Noggin/EGF for growth. Their transcriptome resembles strikingly that of fetal intestinal spheroids, with downregulation of crypt base columnar cell (CBC) markers (Lgr5, Ascl2, Smoc2, Olfm4). In addition, they display upregulation of inflammatory and mesenchymal genetic programs, together with robust expression of YAP target genes. Lineage tracing, cell-sorting and single cell RNA sequencing experiments demonstrate that adult spheroid-generating cells belong to a hitherto undescribed developmental lineage, independent of Lgr5+ve CBCs, and are involved in regeneration of the epithelium following CBC ablation
Published in
A Lgr5-independent developmental lineage is involved in mouse intestinal regeneration
Marefati M, Fernandez-Vallone V, Leprovots M et al. · Development (Cambridge, England) 2025 · PMID 40013494 · doi:10.1242/dev.204654
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Also filed as BioProject PRJNA1092561 and SRA study SRP498106. Searching any of these in the dataset finder brings you back here.

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