GEO series

High-protein diet alleviate cardiac damage, adipose tissue inflammation, and alterations in the gut microbiota induced by chronic sleep fragmentation

GSE262679 Mus musculus (mouse) RNA-seq (gene expression) 13 samples Submitted 2026/03/31 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 13 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 13 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Background: Sleep is fundamental to growth, immune function, and overall health. We initiate our study to elucidate the impact of sleep fragmentation (SF) on the cardiac function, gut microbiome diversity, and the transcriptomic profile of inguinal white adipose tissue (iWAT) in mice, as well as the regulatory role of a high protein diet.

Read the full summary ↓

Methods: We constructed chronic SF and high protein diet intervention mouse models for this research. Cardiac structure and function were evaluated by echocardiographic analyses. Gut microbiota composition was determined by 16s rDNA amplicon sequencing. Transcriptome alterations of iWAT were assessed by RNA-sequencing. Results: Our result revealed that SF interventions induced inflammatory changes in adipose tissue and perturbed the diversity and composition of the gut microbiota. Concurrently, 6-week SF intervention led to a significant decline in left ventricular systolic function in mice, manifested by a notable decrease in EF and FS. Masson staining revealed distinctions compared to the control group, suggesting an increase in myocardial collagen fiber content following SF intervention. High-protein diet intervention partially mitigated the damage to cardiac structure and function caused by SF. Meanwhile, high-protein diet coupled with improvements in the adipose tissue transcriptome changes induced by SF. Conclusions: In conclusion, chronic SF intervention induced cardiac damage, alters gut microbiota composition and induce adipose tissue inflammation. High-protein diet could partially mitigate the changes above.

This dataset

Open GSE262679 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE262679_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1092970 and SRA study SRP498385. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 13 more — browse all 13 samples with per-sample file links →

Datasets on a similar topic

GSE342666 Sodium oxybate, a narcolepsy therapeutic, remodels the gut microbiota and alleviates metabolic dysfunction in developing and established obesity 32 samples shares microbiota, alleviate
GSE307255 HDL-mimetic peptide treatment reverses APOE4-induced transcriptomic and lipidomic alterations in the brain of humanized APOE mice 32 samples shares alterations, induced
GSE319612 Rothia mucilaginosa Alleviates Constipation through Gut Microbiota Remodeling and Neuroactive Pathway Activation 26 samples shares microbiota, alleviate
GSE280782 Preterm birth increases susceptibility to hyperglycemia-induced glomerular alterations in the kidneys of male mice 24 samples shares alterations, induced
GSE312171 Fecal microbiota transplantation mitigates cardiac remodeling and functional impairment in mice with chronic colitis 24 samples shares microbiota, cardiac
GSE280781 Preterm birth increases susceptibility to hyperglycemia-induced glomerular alterations in the kidneys of male mice [bulk RNA-seq] 20 samples shares alterations, induced
GSE291043 PUS10-induced tRNA fragmentation impacts retrotransposon-driven inflammation [Polysome profiling] 18 samples shares fragmentation, induced
GSE286252 Microbiota-indole-3-propionic acid-heart axis mediates the protection of leflunomide against αPD1-induced cardiotoxicity in mice 12 samples shares microbiota, induced

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.