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Transcriptional characterization of mouse and rat ventral pallidum using single nucleus RNA-sequencing

GSE262729 Rattus norvegicus (rat) RNA-seq (gene expression) 8 samples Submitted 2025/10/07 Platform GPL34316Platform GPL24247
At a glance

RNA-seq (gene expression) · rat · 8 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Rattus norvegicus (rat)
Samples
8 samples: 2 × VP rat, vs 2 × NAC rat,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with noncanonical interregional continuityScience advances · 2025
Summary (written by the submitters)
The ventral pallidum (VP) was defined as a basal ganglia nucleus and a major output of the nucleus accumbens. It is known to be involved in motivated behaviors, including feeding. We sought to further characterize VP by sequencing the RNA from individual nuclei in mouse and rat VP and analyzing neuronal expression patterns. We also investigated the impact of a high-fat diet on VP transcription. These data will be useful for understanding cell type diversity in VP, homology between mouse and rat VP, and the impact of diet on VP gene expression.
Published in
Single-cell sequencing of rodent ventral pallidum reveals diverse neuronal subtypes with noncanonical interregional continuity
Ottenheimer DJ, Simon RC, Briones BA et al. · Science advances 2025 · PMID 41337598 · doi:10.1126/sciadv.adp3059
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE262729_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1093069 and SRA study SRP498475. Searching any of these in the dataset finder brings you back here.

Study design
2 × VP rat, vs 2 × NAC rat,

Supports a between-group comparison across 4 samples.

How we read this: 2 replicated groups read from 8 sample titles; they account for 4 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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