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Mechanical unloading triggers glutamine influx and catabolism to suppress osteoclast apoptosis

GSE263705 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/09/09 Platform GPL24247
Summary
External mechanical stimuli are pivotal for the maintenance of bone homeostasis via enhancing bone formation and inhibiting bone resorption. Lack of mechanical loading during prolonged bed rest or exposure to long-term microgravity environment in space leads to a rapid decline in bone mass and bone strength. Osteoblast lineage cells are capable of sensing and transmitting mechanical signals through a variety of pathways. However, the mechanisms by which osteoclasts perceive and respond to mechanical disturbances remain largely unclear. Here, through integrating the data of single-cell transcriptome, metabolome, proteome and ubiquitinome of bone tissues from hindlimb unloading (HLU) and control mice, we clarify that in the absence of mechanical force, osteoclasts are characterized by significant increase in Gln influx and catabolism, as well as suppression of intrinsic apoptosis.
Published in
Integrative Omics Reveals Glutamine Catabolism-Driven Apoptotic Suppression in Monocytes upon Mechanical Unloading
Ding Y, Tong F, Liu M et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 · PMID 40820799 · doi:10.1002/advs.202500585
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Also filed as BioProject PRJNA1091437 and SRA study SRP497543. Searching any of these in the dataset finder brings you back here.

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