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Mechanical confinement prevents ectopic platelet release

GSE264159 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/08/20 Platform GPL30172
Summary
The aim of the project is to identify the genes in megakaryocytes whose expression is modified upon 3D methylcellulose hydrogel culture compared to liquid culture. Megakaryocytes are the precursor cells of platelets. We previously showed that growing megakaryocytes in 3D hydrogel strongly modifies their differentiation and their morphology, being closer to the one in situ. We were thus interested into identifying these mechanobiological pathways induced by the confinement. We found that 3D culture notably upregulated genes related to the Rho GTPase pathway.
Published in
Mechanical confinement prevents ectopic platelet release
Guinard I, Brassard-Jollive N, Ruch L et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 39236232 · doi:10.1073/pnas.2407829121
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Also filed as BioProject PRJNA1101070 and SRA study SRP502111. Searching any of these in the dataset finder brings you back here.

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