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Analysis of gene expression in SLMAP3 knockout immortalized E14.5 mouse embryonic fibroblasts

GSE266611 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/05/08 Platform GPL30172
Summary
Due to the developmental abnormalities observed in SLMAP3 knockout mouse embryos, we aimed to investigated the impact of gene expression in E14.5 mouse embryonic fibroblasts (MEFs) isolated from these animals. The cells used for RNA-sequencing were previously immortalized with a transient expression of SV40 large T (SV40 1: pBSSVD2005 was a gift from David Ron - Addgene plasmid # 21826 ; http://n2t.net/addgene:21826 ; RRID:Addgene_21826). These immortalized cells express mostly the largest isoforms of SLMAP (~ 90kDa), which are the ones knocked out in the embryos, and therefore compensation because of other isoforms is negligible.
Published in
SLMAP3 is crucial for organogenesis through mechanisms involving primary cilia formation
Dias AP, Rehmani T, Applin BD et al. · Open biology 2024 · PMID 39417621 · doi:10.1098/rsob.240206
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Also filed as BioProject PRJNA1107953 and SRA study SRP505778. Searching any of these in the dataset finder brings you back here.

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