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Mmp14-Dependent Remodeling of the Pericellular-Dermal Collagen Interface Governs Fibroblast Survival

GSE266914 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/24 Platform GPL24247
Summary
Dermal fibroblasts deposit type I collagen, the dominant extracellular matrix molecule found in skin, during early postnatal development. Coincident with this biosynthetic program, fibroblasts proteolytically remodel pericellular collagen fibrils by mobilizing the membrane-anchored matrix metalloproteinase, Mmp14. Unexpectedly, dermal fibroblasts in Mmp14-/- mice commit to a large-scale apoptotic program that leaves skin tissues replete with dying cells. A requirement for Mmp14 in dermal fibroblast survival is recapitulated in vitro when cells are embedded within, but not cultured atop, 3-dimensional hydrogels of cross-linked type I collagen. In the absence of Mmp14-dependent pericellular proteolysis, dermal fibroblasts fail to trigger β1 integrin activation and instead actuate a TGF-β1/phospho-JNK stress response that leads to apoptotic cell death in vitro as well as in vivo. Taken together, these studies identify Mmp14 as a requisite cell survival factor that maintains dermal fibroblast viability in postnatal dermal tissues.
Published in
Mmp14-dependent remodeling of the pericellular-dermal collagen interface governs fibroblast survival
Sabeh F, Li XY, Olson AW et al. · The Journal of cell biology 2024 · PMID 38990714 · doi:10.1083/jcb.202312091
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Also filed as BioProject PRJNA1108711 and SRA study SRP506176. Searching any of these in the dataset finder brings you back here.

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