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DHX36-mediated G-quadruplexes unwinding is essential for oocyte and early embryo development in mice

GSE266915 Mus musculus Expression profiling by high throughput sequencing 29 samples 2025/01/29 GPL24247
Summary
The role of G-quadruplex (G4) structures and their effects on oocyte and early embryo development remain unclear. We discovered that the G4 helicase DHX36 is essential for oocyte growth and the maternal-to-zygotic transition (MZT). Conditional knockout of DHX36 resulted in DNA G4 accumulation in mouse oocytes, reducing chromatin accessibility, and inhibiting RNA transcription, ultimately disrupting transcriptome homeostasis during oocyte growth and MZT.
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