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Integrator complex bridges TFIID and RNA polymerase II to maintain pluripotency during development (RNA-Seq)

GSE267772 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/08/08 Platform GPL19057
Summary
The mechanisms by which the expression of pluripotency and Polycomb networks are harmonized to allow the transition from the pluripotency to a differentiated state has not been fully elucidated. Integrator complex regulates transcription pause release and RNA processing in metazoans. Here, we show that acute depletion of INTS11 in mouse embryonic stem cells or its conditional deletion in mouse results in loss of stemness and block of early embryonic development, respectively. We show that Integrator plays a role in stemness and cellular differentiation by bridging the association of RNA polymerase II (RNAPII) and basal transcription factor (TFIID). While the catalytic endonuclease activity is required to enhance cellular reprogramming, depletion of INTS11 diminish RNAPII recruitment to promoters and super-enhancers of pluripotency and Polycomb genes leading to their repression. We place Integrator at pre-initiation complex formation linking the pluripotency and Polycomb pathways to ensure orderly cellular differentiation during development.
Published in
Integrator promotes the association of TFIID and RNA polymerase II to maintain pluripotency during development
Edupuganti RR, Beckedorff F, Cingaram PKR et al. · Molecular cell 2025 · PMID 40780182 · doi:10.1016/j.molcel.2025.07.006
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Also filed as BioProject PRJNA1112842 and SRA study SRP508400. Searching any of these in the dataset finder brings you back here.

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