Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance
RNA-seq (gene expression) · mouse · 26 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 26 samples: 8 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenanceNature communications · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE267913_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1113617 and SRA study SRP508817. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 22 samples.
How we read this: 8 replicated groups read from 26 sample titles; they account for 22 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8281456 AAV_control_1 AAV_control
- GSM8281457 AAV_control_2 AAV_control
- GSM8281458 AAV_control_3 AAV_control
- GSM8281459 AAV_control_4 AAV_control
- GSM8281460 AAV_Etv4_OE_1 AAV_Etv4_OE
- GSM8281461 AAV_Etv4_OE_2 AAV_Etv4_OE
- GSM8281462 AAV_Etv4_OE_3 AAV_Etv4_OE
- GSM8281463 AAV_Lrtm1_OE_1 AAV_Lrtm1_OE
- GSM8281464 AAV_Lrtm1_OE_2 AAV_Lrtm1_OE
- GSM8281465 AAV_Lrtm1_OE_3 AAV_Lrtm1_OE
- GSM8281466 AAV_Pdzrn4_OE_1 AAV_Pdzrn4_OE
- GSM8281467 AAV_Pdzrn4_OE_2 AAV_Pdzrn4_OE
- GSM8281468 AAV_Pdzrn4_OE_3 AAV_Pdzrn4_OE
- GSM8281469 AAV_Pdzrn4_OE_4 AAV_Pdzrn4_OE
- GSM8281470 In_GAS_1 In_GAS
- GSM8281471 In_GAS_2 In_GAS
- GSM8281472 In_TA_1 In_TA
- GSM8281473 In_TA_2 In_TA
- GSM8281474 Den_GAS_1 Den_GAS
- GSM8281475 Den_GAS_2 Den_GAS
- GSM8281476 Den_TA_1 Den_TA
- GSM8281477 Den_TA_2 Den_TA
- GSM8281478 BoTX
- GSM8281479 BoTX_Ctrl
- GSM8281480 MuskKO_Ctrl
- GSM8281481 MuskKO
- GSE267909 Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance (bulk RNA-Seq) 14 samples shares subsynaptic-specific, single-nuclei, neuromuscular, transcripts, maintenance, skeletal, involved, junction
- GSE267910 Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance (snRNA-Seq) 12 samples shares subsynaptic-specific, single-nuclei, neuromuscular, transcripts, maintenance, skeletal, involved, junction
- GSE299537 Inhibition of AhR improves cortical bone and skeletal muscle function via preservation of neuromuscular junctions 16 samples shares neuromuscular, skeletal, junction
- GSE285026 LSMEM2, localized at the neuromuscular junction, modulates mitochondrial integration in skeletal muscles 15 samples shares neuromuscular, skeletal, junction
- GSE303861 The growth factor FGF21 maintains neuromuscular junction through histone deacetylase HDAC4 in denervation-induced skeletal muscle atrophy 4 samples shares neuromuscular, skeletal, junction
- GSE294765 A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy 35 samples shares neuromuscular, skeletal, junction
- GSE210649 Gut microbiota depletion delays peripheral nerve development and impairs neuromuscular junction maturation 36 samples shares neuromuscular, junction
- GSE296707 Neuromuscular junction transcriptome analysis of spinal and bulbar muscular atrophy mice implicates sarcomere gene expression and calcium flux dysregulation in disease pathogenesis 30 samples shares neuromuscular, junction
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.