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Transcriptional analysis of cDC subsets in Irf8+32 -/- and Zeb2 -165(d1+2+3) mice

GSE268380 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/05/29 Platform GPL34290
Summary
To determine if the residual cDC subsets in the Δ1+2+3 and Δ32 mice reflected their normal counterparts, we performed bulk RNA-sequencing analysis of cDC1 and cDC2 from WT, Δ1+2+3 and Δ32 mice. cDC1 from WT and Δ1+2+3 clustered together in principal component analysis (PCA) plot, whereas cDC2 from WT and Δ32 mice cluster together. Further, cDC1 and cDC2 from WT mice show a large number of differentially expressed genes (DEGs), as expected. However, there were few DEGs in cDC1 between WT and Δ1+2+3 mice, or in cDC2 between WT and Δ32 mice, indicating no substantive transcriptional differences between corresponding cDC subsets in WT, Δ32 and Δ1+2+3 mice.
Published in
Shared pathway of WDFY4-dependent cross-presentation of immune complexes by cDC1 and cDC2
Jo S, Ohara RA, Theisen DJ et al. · The Journal of experimental medicine 2025 · PMID 39918736 · doi:10.1084/jem.20240955
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Also filed as BioProject PRJNA1116753 and SRA study SRP509992. Searching any of these in the dataset finder brings you back here.

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