Gene expression analysis of macrophages exposed to LPS and LPS-coated beads
RNA-seq (gene expression) · mouse · 18 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 18 samples: 9 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Absence of c-Maf and IL-10 enables type I IFN enhancement of innate responses to LPS in alveolar macrophagesJournal of immunology (Baltimore, Md. : 1950) · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE268843_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1119031 and SRA study SRP511159. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 18 samples.
How we read this: 9 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8301055 AM, PBS-bead bystander, replicate 1 AM, PBS-bead bystander,
- GSM8301056 AM, PBS-bead positive, replicate 1 AM, PBS-bead positive,
- GSM8301057 AM, LPS-bead bystander, replicate 1 AM, LPS-bead bystander,
- GSM8301058 AM, LPS-bead positive, replicate 1 AM, LPS-bead positive,
- GSM8301059 BMDM, PBS-bead positive, replicate 1 BMDM, PBS-bead positive,
- GSM8301060 BMDM, LPS-bead bystander, replicate 1 BMDM, LPS-bead bystande…
- GSM8301061 BMDM, LPS-bead positive, replicate 1 BMDM, LPS-bead positive,
- GSM8301062 BMDM, untreated, replicate 1 BMDM, untreated,
- GSM8301063 BMDM, soluble LPS, replicate 1 BMDM, soluble LPS,
- GSM8301064 AM, PBS-bead bystander, replicate 2 AM, PBS-bead bystander,
- GSM8301065 AM, PBS-bead positive, replicate 2 AM, PBS-bead positive,
- GSM8301066 AM, LPS-bead bystander, replicate 2 AM, LPS-bead bystander,
- GSM8301067 AM, LPS-bead positive, replicate 2 AM, LPS-bead positive,
- GSM8301068 BMDM, PBS-bead positive, replicate 2 BMDM, PBS-bead positive,
- GSM8301069 BMDM, LPS-bead bystander, replicate 2 BMDM, LPS-bead bystande…
- GSM8301070 BMDM, LPS-bead positive, replicate 2 BMDM, LPS-bead positive,
- GSM8301071 BMDM, untreated, replicate 2 BMDM, untreated,
- GSM8301072 BMDM, soluble LPS, replicate 2 BMDM, soluble LPS,
- GSE201128 The response to lipopolysaccharide (LPS) of bone marrow derived macrophages treated with oxysterol binding protein (OSBP) inhibitors 30 samples shares lps, macrophages
- GSE281503 Effects of daridorexant on gene expression in the hypothalamus of LPS-induced mice. 16 samples shares lps
- GSE325662 Gene expression profiling of lung tissue from LPS-induced acute lung injury mice treated with Renshen Baidu Powder 9 samples shares lps
- GSE264278 TIme-cource single-cell RNA-seq analysis of bleomycin and LPS-induced lung injury and fibrosis 18 samples shares lps
- GSE276682 Single-cell transcriptome analysis revealed cellular reprogramming following Tetramethylpyrazine treatment in LPS-induced acute lung injury 12 samples shares lps
- GSE249970 Transcriptome analysis of lung derived from LPS-induced ARDS model mice treated with EVs or SPLEVs. 4 samples shares lps
- GSE290810 ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits [FACS_SpinalCord_Microglia & LPS_Brain_SpinalCord] 35 samples shares lps
- GSE196249 Secondary LPS stimulation of BMDMs differentiated from bone marrow of C57B/6J mice treated with 4xLPS, 1xLPS or PBS 18 samples shares lps
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.