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Effects of daridorexant on gene expression in the hypothalamus of LPS-induced mice.

GSE281503 Mus musculus (mouse) RNA-seq (gene expression) 16 samples Submitted 2025/02/25 Platform GPL19057
At a glance

RNA-seq (gene expression) · mouse · 16 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
16 samples: 4 × LPS_Vehi vs 4 × LPS_Dari vs 4 × Saline_Vehi vs 4 × Saline_DariGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Orexin Receptor Antagonism Improves Sleep Quality and Mitigates Lipopolysaccharide-Induced Inflammatory Responses in a Mouse ModelFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
Summary (written by the submitters)
Acute inflammatory processes disrupt hypothalamic homeostatic systems, leading to a preference for sleep over wakefulness. Orexin knockout mice exhibit faster recovery from LPS-induced sickness, possibly due to enhanced sleep during inflammation. The reduction in orexin activity limits physical activity and encourages sleep, though it remains uncertain whether these responses are pathological or protective.
Read the full summary ↓
In this study, to investigate the effects of daridorexant, a dual orexin receptor antagonist, on brain inflammation and sleep quality, we employed a systemic inflammatory model in C57BL/6J mice. LPS treatment resulted in significant decreases in REM sleep and wakefulness, while non-REM sleep increased. Here, we provide gene expression profiling of hypothalamus tissues using the RNA sequencing for identifying changes by daridorexant in gene expression of the LPS-treated mice.
Published in
Orexin Receptor Antagonism Improves Sleep Quality and Mitigates Lipopolysaccharide-Induced Inflammatory Responses in a Mouse Model
Horiuchi D, Irukayama-Tomobe Y, Kim JD et al. · FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2026 · PMID 41511173 · doi:10.1096/fj.202502960R
This dataset
Open GSE281503 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE281503_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1184153 and SRA study SRP544232. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41511173) ↗
Study design
4 × LPS_Vehi vs 4 × LPS_Dari vs 4 × Saline_Vehi vs 4 × Saline_Dari

Supports a between-group comparison across 16 samples.

How we read this: 4 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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