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The molecular basis of lamin- chromatin interactions [RNA-Seq]

GSE268922 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/05/28 Platform GPL30172
Summary
Lamins are the main constituents of the nuclear lamina, a fibrillar layer underlyingthe inner nuclear membrane. The nuclear lamina governs chromatinorganization through lamina-associated domainswithin the densely packed heterochromatin regions. Employing cryo-focused ion beam (cryo-FIB) milling in conjunction withcryo-electron tomography (cryo-ET), we mappedthe concentration of nucleosomesat the lamin-chromatin interface. Depletion of lamin A/C induces nanometer-scale chromatin decompaction, macroscopic-scale alterations in gene expression and chromatin-wide alterations of chromatin properties, revealed by 4f-genome-wide analysis. Cryo-electron microscopy (cryo-EM) structural analysis revealed a specific interaction between nucleosomes and the tail domain of lamin A. A unique motif of lamin A that distinguishes it from other lamin isoforms. These findings illuminatethe dynamic interplay between specific lamins and chromatin, shaping chromatin architecture and epigenetic regulation.
Published in
The molecular basis of lamin-specific chromatin interactions
Wang B, Kronenberg-Tenga R, Rosti V et al. · Nature structural & molecular biology 2025 · PMID 40750945 · doi:10.1038/s41594-025-01622-5
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Also filed as BioProject PRJNA1119466 and SRA study SRP511361. Searching any of these in the dataset finder brings you back here.

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