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Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation

GSE269397 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2024/08/14 Platform GPL17021
Summary
The lymphatic system is formed during embryonic development by the commitment of specialized lymphatic endothelial cells (LECs) and their subsequent assembly in primary lymphatic vessels. While lymphatic cells are in continuous contact with mesenchymal cells during development and in adult tissues, the role of mesenchymal cells in lymphatic vasculature development remains poorly characterized. Here, we show that a subpopulation of mesenchymal cells expressing the transcription factor Osr1 are in close association with migrating LECs and established lymphatic vessels in mice. Lineage tracing experiments revealed that Osr1+ cells precede LEC arrival during lymphatic vasculature assembly in the back of the embryo. Using Osr1-deficient embryos and functional in vitro assays, we show that Osr1 acts in a non-cell autonomous manner controlling proliferation and early migration of LECs to peripheral tissues. Thereby, mesenchymal Osr1+ cells control in a bimodal manner the production of extracellular matrix scaffold components and signal ligands critical for lymphatic vessel formation.
Published in
Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation
Vallecillo-García P, Kühnlein MN, Orgeur M et al. · Development (Cambridge, England) 2024 · PMID 39221968 · doi:10.1242/dev.202747
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Also filed as BioProject PRJNA1121373 and SRA study SRP512490. Searching any of these in the dataset finder brings you back here.

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