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RNA-seq analysis of bead-purified B cells of wild-type BALB/c mice stimulated in vitro with TLR9 agonists (CpG ODN 1826, Hokkaido System Science) or left unstimulated.

GSE269458 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/12/31 Platform GPL24247
Summary
The goal of this study was to develop a B-cell specific gene signature to catalog induced genes after short term in vitro TLR9 stimulation of wild-type BALB/c B cells. B cells were bead purified from splenocytes (two spleens combined for one replicate) of 7-11-week-old wild-type BALB/c mice. The bead purified B cells (10 million (M) per replicate) were stimulated for 4 hours at 10M/ml with TLR9 agonist CpG (CpG ODN 1826, Hokkaido System Science) at 10µg/ml or left unstimulated. RNA was isolated using the RNeasy Plus Mini Kit (QIAGEN). Samples were sequenced on an NextSeq500 (Illumina, Inc) with 2 x 75 bp paired-end reads (20 million reads per sample). B-cell specific gene signature of induced genes after short term in vitro TLR9 stimulation was cataloged.
Published in
Divergent TIR signaling domains in TLR7 and TLR9 control opposing effects on systemic autoimmunity
Leibler C, Thomas KB, Josensi C et al. · The Journal of clinical investigation 2025 · PMID 40794441 · doi:10.1172/JCI189566
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Direct links to NCBI, no account and no request form: the whole study as GSE269458_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1122066 and SRA study SRP512846. Searching any of these in the dataset finder brings you back here.

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