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Effects of different temperatures on chondrocyte growth: a transcriptomic analysis

GSE269585 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2024/12/10 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 4 × 37C vs 4 × 40C vs 4 × 44CGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Effects of different temperatures on chondrocyte growth: a transcriptomic analysisBMC medical genomics · 2024
Summary (written by the submitters)
BACKGROUND: We previously demonstrated that temperature-related microwave ablation (MWA) can safely regulate vertebral growth plates of piglets. Therefore, the present study was designed to investigate the effects of different temperatures on chondrocyte proliferation and the molecular mechanisms involved in vitro. METHODS: After treatment for 10 min using different temperatures (37°C, 40°C, 42°C, 44°C, 46°C, 48°C, or 50°C), CCK-8 assay examined the activity of ATDC5 cells at 12 hr.
Read the full summary ↓
RNA-seq identified differentially expressed genes (DEGs) and the hub genes in ATDC5 cells treated at 37°C, 40°C and 44°C. RT-qPCR verified the differential expression of hub genes in ATDC5 cells. RESULTS: Compared with 37°C, stimulation at 40°C significantly enhanced the activity of ATDC5 cells, and 40°C had no significant effect. In addition, stimulation at 44°C, 46°C, 48°C, or 50°C exhibited the opposite pattern, and ATDC5 cells were particularly less than 50% active after treatment at 46°C, 48°C, or 50°C. Differential expression analysis revealed the presence of 179, 374 and 221 DEGs in 40°C vs. 37°C, 44°C vs. 37°C, and 44°C vs. 40°C, respectively. Enrichment analysis indicated that these DEGs predominantly regulate proliferation, differentiation, necrosis, inflammatory and immune responses, and ECM synthesis/degradation, and that they are associated with the Ras, PI3K/AKT, mTOR, cAMP, and MAPK pathways. Furthermore, Agt, Hspa1a, Hspb1, and Nlrc4 were hub genes in DEGs, and RT-qPCR confirmed that the mRNA expression patterns of these hub genes in ATDC5 cells were essentially consistent with the RNA-seq results. CONCLUSION: Temperature-mediated chondrocyte proliferation was associated with Ras, PI3K/AKT, mTOR, cAMP, and MAPK pathways, and Agt, Hspa1a, Hspb1, and Nlrc4 may be the key regulatory genes.
Published in
Effects of different temperatures on chondrocyte growth: a transcriptomic analysis
Zhao W, Wang Y, Xie J et al. · BMC medical genomics 2024 · PMID 39707345 · doi:10.1186/s12920-024-02070-8
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE269585_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1122570 and SRA study SRP513234. Searching any of these in the dataset finder brings you back here.

Study design
4 × 37C vs 4 × 40C vs 4 × 44C

Supports a between-group comparison across 12 samples.

How we read this: 3 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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