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GLS2 links glutamine metabolism and atherosclerosis by remodelling artery walls (MOVAS).

GSE270056 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2024/10/17 Platform GPL17021
Summary
Metabolic dysregulation, including perturbed glutamine-glutamate homeostasis, is common in atherosclerotic cardiovascular disease. Here, we reveal that modulation of glutaminolysis or glutamate availability in culture media is critical for smooth muscle cell line (MOVAS) phenotypic switching. Cells were treated for 24 hours with glutaminase inhibitors (50mM DON, Sigma-SML0601) in presence or absence of 2mM glutamate suplementation (Gibco-35050061). High-throughput transcriptional profiling revealed that modulation of glutamine and glutamate homeostasis impacted genes involved in protein and extracellular matrix organization, cell motility and microtubule. Thus, we uncovered a novel role for glutamine metabolism in the phenotypic switch in SMCs, a hallmark of vascular remodelling.
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Also filed as BioProject PRJNA1124916 and SRA study SRP514395. Searching any of these in the dataset finder brings you back here.

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