GEO series

5G-exposed human skin cells do not respond with altered gene expression and methylation profiles [RNA-Seq]

GSE271704 Homo sapiens (human) DNA methylation 120 samples Submitted 2025/05/01 Platform GPL24676

At a glance

DNA methylation · human · 120 samples

What it is: DNA methylationChemical marks on DNA that help switch genes on or off.
Organism: Homo sapiens (human)
Samples: 120 samples: 120 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: 5G-exposed human skin cells do not respond with altered gene expression and methylation profilesPNAS nexus · 2025

Summary (written by the submitters)

Due to the ever-increasing wirelessly transmitted data, the development of new transmission standards and higher frequencies in the 5G band is required. Despite basic biophysical considerations that argue against health effects, there is public concern about this technology. Because the skin penetration depth at these frequencies is only 1 millimeter or less, we exposed fibroblasts and keratinocytes to electromagnetic fields up to ten times the permissible limits, for 2 and 48 hours in a fully blinded experimental design.

Read the full summary ↓

Sham-exposed cells served as negative, and UV-exposed cells as positive controls. Differences in gene expression and methylation due to exposure were small and not higher than expected by chance. These data strongly support the assessment that there is no evidence for exposure-induced damage to human skin cells.

Published in

5G-exposed human skin cells do not respond with altered gene expression and methylation profiles

Jyoti J, Gronau I, Cakir E et al. · PNAS nexus 2025 · PMID 40365161 · doi:10.1093/pnasnexus/pgaf127

This dataset

Open GSE271704 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE271704_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for beta-value tables or raw IDAT files among the supplementary files.

Also filed as BioProject PRJNA1132959 and SRA study SRP518496. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40365161) ↗

Study design

120 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 120 sample titles: 120 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8382919 Exp-V1K_2A,Cell-HaCaT,Power-1,Frequency-27,Time-48,Treatment-sham
GSM8382920 Exp-V1K_2B,Cell-HaCaT,Power-1,Frequency-27,Time-48,Treatment-exposed
GSM8382921 Exp-V1K_3A,Cell-HaCaT,Power-1,Frequency-27,Time-48,Treatment-sham
GSM8382922 Exp-V1K_3B,Cell-HaCaT,Power-1,Frequency-27,Time-48,Treatment-exposed
GSM8382923 Exp-V1K_4A,Cell-HaCaT,Power-1,Frequency-27,Time-48,Treatment-exposed
GSM8382924 Exp-V1K_4B,Cell-HaCaT,Power-1,Frequency-27,Time-48,Treatment-sham
GSM8382925 Exp-V2K_4A,Cell-HaCaT,Power-1,Frequency-27,Time-2,Treatment-exposed
GSM8382926 Exp-V2K_4B,Cell-HaCaT,Power-1,Frequency-27,Time-2,Treatment-sham
GSM8382927 Exp-V2K_5A,Cell-HaCaT,Power-1,Frequency-27,Time-2,Treatment-sham
GSM8382928 Exp-V2K_5B,Cell-HaCaT,Power-1,Frequency-27,Time-2,Treatment-exposed
GSM8382929 Exp-V2K_6A,Cell-HaCaT,Power-1,Frequency-27,Time-2,Treatment-exposed
GSM8382930 Exp-V2K_6B,Cell-HaCaT,Power-1,Frequency-27,Time-2,Treatment-sham
GSM8382931 Exp-V3K_1A,Cell-HaCaT,Power-1,Frequency-40.5,Time-2,Treatment-sham
GSM8382932 Exp-V3K_1B,Cell-HaCaT,Power-1,Frequency-40.5,Time-2,Treatment-exposed
GSM8382933 Exp-V3K_3A,Cell-HaCaT,Power-1,Frequency-40.5,Time-2,Treatment-sham
GSM8382934 Exp-V3K_3B,Cell-HaCaT,Power-1,Frequency-40.5,Time-2,Treatment-exposed
GSM8382935 Exp-V3K_4A,Cell-HaCaT,Power-1,Frequency-40.5,Time-2,Treatment-exposed
GSM8382936 Exp-V3K_4B,Cell-HaCaT,Power-1,Frequency-40.5,Time-2,Treatment-sham
GSM8382937 Exp-V4K_1A,Cell-HaCaT,Power-1,Frequency-40.5,Time-48,Treatment-exposed
GSM8382938 Exp-V4K_1B,Cell-HaCaT,Power-1,Frequency-40.5,Time-48,Treatment-sham
GSM8382939 Exp-V4K_3A,Cell-HaCaT,Power-1,Frequency-40.5,Time-48,Treatment-exposed
GSM8382940 Exp-V4K_3B,Cell-HaCaT,Power-1,Frequency-40.5,Time-48,Treatment-sham
GSM8382941 Exp-V4K_4A,Cell-HaCaT,Power-1,Frequency-40.5,Time-48,Treatment-sham
GSM8382942 Exp-V4K_4B,Cell-HaCaT,Power-1,Frequency-40.5,Time-48,Treatment-exposed
GSM8382943 Exp-V2H_1A,Cell-HDF,Power-1,Frequency-40.5,Time-2,Treatment-sham
GSM8382944 Exp-V2H_1B,Cell-HDF,Power-1,Frequency-40.5,Time-2,Treatment-exposed
GSM8382945 Exp-V2F_2A,Cell-HDF,Power-1,Frequency-40.5,Time-2,Treatment-sham
GSM8382946 Exp-V2F_2B,Cell-HDF,Power-1,Frequency-40.5,Time-2,Treatment-exposed
GSM8382947 Exp-V2F_3A,Cell-HDF,Power-1,Frequency-40.5,Time-2,Treatment-sham
GSM8382948 Exp-V2F_3B,Cell-HDF,Power-1,Frequency-40.5,Time-2,Treatment-exposed
GSM8382949 Exp-V3F_1A,Cell-HDF,Power-1,Frequency-27,Time-2,Treatment-exposed
GSM8382950 Exp-V3F_1B,Cell-HDF,Power-1,Frequency-27,Time-2,Treatment-sham
GSM8382951 Exp-V3F_2A,Cell-HDF,Power-1,Frequency-27,Time-2,Treatment-exposed
GSM8382952 Exp-V3F_2B,Cell-HDF,Power-1,Frequency-27,Time-2,Treatment-sham
GSM8382953 Exp-V3F_3A,Cell-HDF,Power-1,Frequency-27,Time-2,Treatment-exposed
GSM8382954 Exp-V3F_3B,Cell-HDF,Power-1,Frequency-27,Time-2,Treatment-sham
GSM8382955 Exp-V1F_2A,Cell-HDF,Power-1,Frequency-40.5,Time-48,Treatment-exposed
GSM8382956 Exp-V1F_2B,Cell-HDF,Power-1,Frequency-40.5,Time-48,Treatment-sham
GSM8382957 Exp-V1F_3A,Cell-HDF,Power-1,Frequency-40.5,Time-48,Treatment-exposed
GSM8382958 Exp-V1F_3B,Cell-HDF,Power-1,Frequency-40.5,Time-48,Treatment-sham

+ 80 more — browse all 120 samples with per-sample file links →

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