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RNA-seq analysis of EpCAM-GFP and GFP overexpression in FLO-1 esophageal adenocarcinoma

GSE272221 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/01/13 Platform GPL28038
Summary
EpCAM is one of the highly expressed marker in esophageal adenocarcinoma as identidied in several multi-OMICS platform. We utilize FLO-1 cells which are lacking in EpCAM expresssion and use overexpression strategy to elucidate the function of EpCAM in this cells. Recombinant EpCAM-GFP or GFP only was trasnfected in FLO-1 cells, and the stable cells after antibiotic selection were isolated and expanded. The cells were then subjected to mRNA sequencing.
Published in
RNA-Seq analysis reveals regulatory networks driven by EpCAM overexpression in esophageal adenocarcinoma cells
Mohtar MA, Nasir SN, Abd Razak Z et al. · PeerJ 2026 · PMID 41769390 · doi:10.7717/peerj.20877
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Also filed as BioProject PRJNA1135884 and SRA study SRP519863. Searching any of these in the dataset finder brings you back here.

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