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Comprehensive analysis of differentially expressed lncRNA, and mRNA and their ceRNA networks in mice with the initial stage acute pancreatitis

GSE272464 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/11/13 Platform GPL21273
Summary
Acute pancreatitis (AP) is a prevalent digestive disorder with significant morbidity and mortality rates. This study, for the first time, employed high-throughput sequencing on pancreatic tissues from normal and AP mice to identify differentially expressed long noncoding RNAs (lncRNAs) and mRNAs, as well as related signaling pathways, in the early stage of AP. We found 261 lncRNAs and 1,522 mRNAs to be differentially expressed, with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealing gene functions enriched in cellular metabolism, intercellular structure, and positive regulation of inflammation. By constructing lncRNA-miRNA-mRNA networks, we explored underlying mechanisms and interactions in AP. Notably, knockout of Neat1 in mice inhibited pyroptosis in AP and severe acute pancreatitis (SAP) models, suggesting Neat1's pivotal role in AP pathogenesis. These findings highlight the importance of lncRNAs, particularly Neat1, in AP, offering new insights into SAP pathogenesis and potential therapeutic targets.
Published in
Identifying the ceRNA Regulatory Network in Early-Stage Acute Pancreatitis and Investigating the Therapeutic Potential of NEAT1 in Mouse Models
Lin B, Huang C · Journal of inflammation research 2024 · PMID 39507263 · doi:10.2147/JIR.S490315
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Direct links to NCBI, no account and no request form: the whole study as GSE272464_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1136914 and SRA study SRP520554. Searching any of these in the dataset finder brings you back here.

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