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Early life stress modulates neonatal somatosensation and the transcriptional profile of immature sensory neurons

GSE272525 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/03/26 Platform GPL30172
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Mouse DRG, P9, Control, Female vs 3 × Mouse DRG, P9, Control, Male vs 3 × Mouse DRG, P9, Stress, Female vs 3 × Mouse DRG, P9, Stress, MaleGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Early life stress modulates neonatal somatosensation and the transcriptional profile of immature sensory neuronsPain · 2025
Summary (written by the submitters)
Early life stress (ELS) is associated with an increased risk of experiencing chronic pain during adulthood, but surprisingly little is known about the short-term influence of ELS on nociceptive processing in the immature nervous system and the concomitant effects on somatosensation in the neonate. Here we investigate how ELS modulates pain in neonatal mice as well as the transcriptional and electrophysiological signatures of immature dorsal root ganglia (DRG).
Read the full summary ↓
Shortly after the administration of a neonatal limiting bedding (NLB) paradigm from postnatal days (P)2 to P9, both male and female pups exhibited robust hyperalgesia in response to mechanical, pressure and noxious cold stimuli compared to a control group housed under standard conditions, with no change in their sensitivity to noxious heat. Bulk RNA-seq analysis of L3-L5 DRGs at P9 revealed significant alterations in the transcription of pain- and itch-related genes following ELS, highlighted by a marked downregulation in Sst, Nppb, Chrna6, Trpa1 and Il31ra. Nonetheless, ex vivo whole cell patch-clamp recordings from putative A- and C-fiber sensory neurons in the neonatal DRG found no significant changes in their intrinsic membrane excitability following NLB. Overall, these findings suggest that ELS triggers hyperalgesia in neonates across multiple pain modalities that is accompanied by transcriptional plasticity within developing sensory neurons. A better understanding of the mechanisms governing the interactions between chronic stress and pain during the neonatal period could inform the future development of novel interventional strategies to relieve pain in infants and children who have experienced trauma.
Published in
Early life stress modulates neonatal somatosensation and the transcriptional profile of immature sensory neurons
Harbour K, Eid F, Serafin E et al. · Pain 2025 · PMID 40106369 · doi:10.1097/j.pain.0000000000003416
This dataset
Open GSE272525 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE272525_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1137297 and SRA study SRP520743. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40106369) ↗
Study design
3 × Mouse DRG, P9, Control, Female vs 3 × Mouse DRG, P9, Control, Male vs 3 × Mouse DRG, P9, Stress, Female vs 3 × Mouse DRG, P9, Stress, Male

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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