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RNA-Seq of mGULL knockdown and hGULL rescue in C57BL6 wild-type mice

GSE272715 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2025/04/09 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The functionally conserved human lncRNA motif GULF lowers glucose and lipid levels in obese miceThe Journal of clinical investigation · 2025
Summary (written by the submitters)
For determined h/mGULL have the function similarities, we employed rescue experiments in mice. We knocked down mGULL in mouse liver using shRNAs delivered by adenovirus, and subsequently co-expressed hGULL to assess its capacity to rescue for the loss of function effect of mGULL. After confirmed the mGULL knockdown or hGULL overexpression efficiency, we isolation the total RNA for RNA seq.
Published in
The functionally conserved human lncRNA motif GULF lowers glucose and lipid levels in obese mice
Li Z, Seok S, Jiang C et al. · The Journal of clinical investigation 2025 · PMID 40955662 · doi:10.1172/JCI186355
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE272715_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1138250 and SRA study SRP521159. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 12 more — browse all 12 samples with per-sample file links →

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