Overlapping and distinctfunctions of SPT6, PNUTS and PCF11 in regulating transcription termination [RNA-Seq]
RNA-seq (gene expression) · human · 21 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 21 samples: 7 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Overlapping and distinct functions of SPT6, PNUTS, and PCF11 in regulating transcription terminationNucleic acids research · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE272848_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1139116 and SRA study SRP521586. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 21 samples.
How we read this: 7 replicated groups read from 21 sample titles; they account for 21 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8413585 HeLa, siCT, rep1 HeLa, siCT,
- GSM8413586 HeLa, siCT, rep2 HeLa, siCT,
- GSM8413587 HeLa, siCT, rep3 HeLa, siCT,
- GSM8413588 HeLa, siSPT6, rep1 HeLa, siSPT6,
- GSM8413589 HeLa, siSPT6, rep2 HeLa, siSPT6,
- GSM8413590 HeLa, siSPT6, rep3 HeLa, siSPT6,
- GSM8413591 HeLa, siIWS1, rep1 HeLa, siIWS1,
- GSM8413592 HeLa, siIWS1, rep2 HeLa, siIWS1,
- GSM8413593 HeLa, siIWS1, rep3 HeLa, siIWS1,
- GSM8413594 HeLa, siPNUTS, rep1 HeLa, siPNUTS,
- GSM8413595 HeLa, siPNUTS, rep2 HeLa, siPNUTS,
- GSM8413596 HeLa, siPNUTS, rep3 HeLa, siPNUTS,
- GSM8413597 HeLa, siPCF11, rep1 HeLa, siPCF11,
- GSM8413598 HeLa, siPCF11, rep2 HeLa, siPCF11,
- GSM8413599 HeLa, siPCF11, rep3 HeLa, siPCF11,
- GSM8413600 HeLa, siSPT6siPNUTS, rep1 HeLa, siSPT6siPNUTS,
- GSM8413601 HeLa, siSPT6siPNUTS, rep2 HeLa, siSPT6siPNUTS,
- GSM8413602 HeLa, siSPT6siPNUTS, rep3 HeLa, siSPT6siPNUTS,
- GSM8413603 HeLa, siSPT6siPCF11, rep1 HeLa, siSPT6siPCF11,
- GSM8413604 HeLa, siSPT6siPCF11, rep2 HeLa, siSPT6siPCF11,
- GSM8413605 HeLa, siSPT6siPCF11, rep3 HeLa, siSPT6siPCF11,
- GSE272847 Overlapping and distinctfunctions of SPT6, PNUTS and PCF11 in regulating transcription termination [ChIP-Seq] 11 samples shares pnuts, pcf11, spt6, distinctfunctions, transcription, overlapping, termination, regulating
- GSE269357 PP1 PNUTS binds the “restrictor” and dephosphorylates pol II CTD Ser5 to stimulate RNA pol II transcription termination [nascent RNA-seq] 21 samples shares pnuts, transcription, termination
- GSE269359 PP1 PNUTS binds the “restrictor” and dephosphorylates pol II CTD Ser5 to stimulate RNA pol II transcription termination [ChIP-seq] 22 samples shares pnuts, transcription, termination
- GSE254148 m6Am sequesters PCF11 to promote full-length transcription [RNA-seq] 6 samples shares pcf11, transcription
- GSE234137 m6Am sequesters PCF11 to promote full-length transcription 99 samples shares pcf11, transcription
- GSE234131 m6Am sequesters PCF11 to promote full-length transcription [PCT] 18 samples shares pcf11, transcription
- GSE233877 m6Am sequesters PCF11 to promote full-length transcription [ChIP-Seq] 14 samples shares pcf11, transcription
- GSE234133 m6Am sequesters PCF11 to promote full-length transcription [anabolicSLAM] 8 samples shares pcf11, transcription
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.