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Chromatin remodeler CHD4 establishes chromatin states required for ovarian reserve formation, maintenance, and germ cell survival [ATAC-seq]

GSE273308 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2024/09/27 Platform GPL21273Platform GPL34290
Summary
To understand the functional role of CHD4, a member of the Nucleosome Remodeling and Deacetylase (NuRD) complex, in establishing chromatin states in the formation and maintenance of ovarian reserve and the maintenance of female and male germ cells, we eliminated CHD4 function in germ cells using Ddx4-Cre and Gdf9-iCre to generate CHD4 conditional knockout (Chd4 DcKO and GcKO) mice. We discover that CHD4 defines the chromatin state that ensures germ cell survival, thereby enabling the long-term maintenance of female and male germ cells. We then performed ATAC-seq in Chd4 Dctrl and Chd4 DcKO non-growing oocytes and undifferentiated spermatogonia.
Published in
Chromatin remodeler CHD4 establishes chromatin states required for ovarian reserve formation, maintenance, and germ cell survival
Munakata Y, Hu M, Kitamura Y et al. · bioRxiv : the preprint server for biology 2024 · PMID 39185217 · doi:10.1101/2024.08.12.607691
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Also filed as BioProject PRJNA1141403 and SRA study SRP522801. Searching any of these in the dataset finder brings you back here.

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