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The therapeutic mechanism of baritinib in idiopathic granulomatous mastitis treatment elucidated by single-cell RNA sequencing

GSE274011 Homo sapiens (human) Single-cell RNA-seq 13 samples Submitted 2025/04/15 Platform GPL29480
At a glance

Single-cell RNA-seq · human · 13 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Homo sapiens (human)
Samples
13 samples: 5 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
The pathogenesis of idiopathic granulomatous mastitis (IGM) remains poorly understood. Based on compelling clinical evidence, we have discovered that baricitinib, a widely prescribed medication for autoimmune disorders, exhibits remarkable efficacy in alleviating the symptoms associated with IGM. To gain a more comprehensive understanding of the underlying mechanism of baricitinib in IGM, we performed single-cell RNA sequencing (scRNA-seq) analysis and observed an augmented presence of lip-macrophages and plasma cells_IGHG in patients with IGM.
Read the full summary ↓
Our results indicated that IGM may possess autoimmune characteristics, potentially triggered by an upregulation of plasma cells. And plasma cells were undergoing class-switch recombination from IGHA to IGHG to lead to development of autoimmunity in IGM. Our data also revealed that lip-macrophages involved in maintaining breast homeostasis are associated with pathways related to phagocytosis, endocytosis, and lipid metabolism
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274011_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1144462 and SRA study SRP524262. Searching any of these in the dataset finder brings you back here.

Study design
5 conditions, mostly in duplicate
Micorwell-seq datasets of patient4 ×4 Micorwell-seq datasets of patient3 ×3 Micorwell-seq datasets of patient1 ×2 Micorwell-seq datasets of patient2 ×2 Micorwell-seq datasets of patient5 ×2

Supports a between-group comparison across 13 samples.

How we read this: 5 replicated groups read from 13 sample titles; they account for 13 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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