← BioTransfer GEO Dataset Finder
GEO series

TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

GSE274465 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 12 samples Submitted 2025/05/27 Platform GPL24676
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 12 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Cell cycle-dependent TICRR/TRESLIN and MTBP chromatin binding mechanisms and patternsGenome biology · 2025
Summary (written by the submitters)
The selection of replication origins plays a crucial role in eukaryotic DNA replication. However, the mechanisms of origin selection in humans still need to be defined. Recent work in yeast has shown that the recruitment of Sld3-Sld7 to origins during G1 is involved in origin selection. In humans, TICRR/TRESLIN and MTBP are the orthologs of Sld3 and Sld7, respectively. In this study, we have mapped the genomic binding locations of TICRR/TRESLIN and MTBP in cells where loading of MCM2-7 complex has been inhibited by dox-inducible expression of a degradation-resistant Geminin(T25D).
Published in
Cell cycle-dependent TICRR/TRESLIN and MTBP chromatin binding mechanisms and patterns
Noble TD, Sansam CG, Wittig KA et al. · Genome biology 2025 · PMID 40624716 · doi:10.1186/s13059-025-03665-9
This dataset
Open GSE274465 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274465_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1146464 and SRA study SRP525432. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40624716) ↗
Study design
6 conditions, mostly in duplicate
HCT116, thymidine-mimosine-NoDoxControl, anti… ×2 HCT116, thymidine-mimosine-doxycycline, anti-… ×2 MTBP, thymidine-mimosine-NoDoxControl, anti-G… ×2 MTBP, thymidine-mimosine-doxycycline, anti-GF… ×2 TICRR, thymidine-mimosine-NoDoxControl, anti-… ×2 TICRR, thymidine-mimosine-doxycycline, anti-G… ×2

Supports a between-group comparison across 12 samples.

How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.