GEO series

Smug1 DNA glycosylase modulates the perception of smell in mouse brain through downregulation of olfactory receptor genes

GSE274703 Mus musculus (mouse) RNA-seq (gene expression) 30 samples Submitted 2025/10/20 Platform GPL21103

At a glance

RNA-seq (gene expression) · mouse · 30 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 30 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: SMUG1 DNA glycosylase modulates reward behaviour through regulation of olfactory receptor gene expressionNucleic acids research · 2025

Summary (written by the submitters)

The SMUG1-DNA glycosylase is the primary enzyme responsible for excising 5-hydroxymethyluracil (5hmU) from genomic DNA. SMUG1 activity is high in the brain and is therefore proposed to be particularly important for regulating 5hmU levels in the brain. 5hmU has been proposed as an epigenetic mark, but it is still unclear whether, and in which contexts, it may have regulatory functions.

Read the full summary ↓

Here we show that accumulation of 5hmU in Smug1 deficient mice leads to widespread gene expression alterations across multiple brain regions, and that this is accompanied by a complex behavioural phenotype. We demonstrate that 5hmU serves as a transcriptional regulator of olfactory receptor genes in the olfactory bulb. The persistence of 5hmU in the genome leads to deficits in sensory perception of smell and influences reward-related behaviour. Mechanistically, we identify a specific function for SMUG1 in removing 5hmU from promoters of olfactory receptor genes, promoting transcription and proper olfaction function.

Published in

SMUG1 DNA glycosylase modulates reward behaviour through regulation of olfactory receptor gene expression

Bordin DL, Lefol Y, Largartos-Donate MJ et al. · Nucleic acids research 2025 · PMID 40716776 · doi:10.1093/nar/gkaf722

This dataset

Open GSE274703 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274703_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1147682 and SRA study SRP526189. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40716776) ↗

Samples in this study

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+ 30 more — browse all 30 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.