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Enterocyte dedifferentiation drives colonic regeneration following irradiation injury [scRNA-seq]

GSE274805 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/08/16 Platform GPL21273
Summary
The intestinal epithelium consistently exposes to various injuries and exhibits remarkable regenerative capacity. Although small intestinal regeneration has been extensively investigated, the mechanisms governing colonic regeneration remain poorly understood. Here, we identify irradiation-induced regenerative cells (IRECs), a previously unrecognized colonic enterocyte population, emerges following irradiation injury. IRECs, marked by the gene YuanShangCao (Ysc), undergo dedifferentiation and transition into a stem-like state, contributing to epithelial regeneration. IREC ablation severely impairs colonic repair. Mechanistically, YSC promotes colonic regeneration by preventing the lysosomal degradation of YAP. Furthermore, we demonstrate a critical role of p53 in colonic regeneration by inducing Ysc expression. Together, our findings define IRECs as an irradiation injury-induced enterocyte population that dedifferentiate into stem cells in colon epithelial repair and establish the p53-YSC-YAP axis plays a key role in this process, deepening the understanding of colonic diseases.
Published in
Crotonate enhances intestinal regeneration after injury via HBO1-mediated H3K14 crotonylation
Xiao Y, Yu S, Zhang M et al. · Nature communications 2025 · PMID 41038843 · doi:10.1038/s41467-025-63869-2
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Also filed as BioProject PRJNA1148083 and SRA study SRP526483. Searching any of these in the dataset finder brings you back here.

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