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When glucocorticoid receptor meets the mineralocorticoid receptor in human cells

GSE275128 Homo sapiens (human) RNA-seq (gene expression) 80 samples Submitted 2025/06/16 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 80 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
80 samples: 20 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functionsThe Journal of biological chemistry · 2025
Summary (written by the submitters)
Glucocorticoids are widely used to treat a variety of inflammatory diseases. Mechanistically glucocorticoid ligands induce glucocorticoid receptor (GR) homodimerization and regulation of gene expression. Here we show that GR and mineralocorticoid receptor (MR) form molecular complexes with distinct transcriptional responses that alter the biological roles of GR. MR inhibited GR interaction with genomic DNA and diminished glucocorticoid-regulated gene expression as well as suppressed cell apoptosis induced by GR signaling.
Published in
Molecular interactions of glucocorticoid and mineralocorticoid receptors define novel transcription and biological functions
Sueyoshi T, Petrillo MG, Jewell CM et al. · The Journal of biological chemistry 2025 · PMID 40209952 · doi:10.1016/j.jbc.2025.108488
This dataset
Open GSE275128 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275128_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1149651 and SRA study SRP527271. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40209952) ↗
Study design
20 conditions, mostly with about 4 replicates each
Set1-off-veh ×4 Set1-off-Dex1nm ×4 Set1-off-Aldo1nm ×4 Set1-GR-veh ×4 Set1-GR-Dex1nm ×4 Set1-GR-Aldo1nm ×4 Set1-MR-veh ×4 Set1-MR-Dex1nm ×4 +12 more

Supports a between-group comparison across 80 samples.

How we read this: 20 replicated groups read from 80 sample titles; they account for 80 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 40 more — browse all 80 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.