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10X single-cell RNA-seq profiling of ccRCC model tumors and normal kidney tissue

GSE275339 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/09/20 Platform GPL24247
Summary
To achieve a robust immunocompetent mouse model of human ccRCC, we directly injected adenovirus-associated viruses (AAVs) encoding sgRNAs against VHL and other known/suspected ccRCC tumor suppressor genes into the kidneys of C57BL/6 mice under conditions where Cas9 was under the control of the Pax8 promoter to induce kidney tumors. An AAV targeting Vhl, Pbrm1, Keap1 and Tsc1 reproducibly caused macroscopic ccRCCs that partially resembled human ccRCC tumors with respect to transcriptome and cell of origin and responded to a ccRCC standard of care agent, axitinib.
Published in
Toward a CRISPR-based mouse model of Vhl-deficient clear cell kidney cancer: Initial experience and lessons learned
Stransky LA, Gao W, Schmidt LS et al. · Proceedings of the National Academy of Sciences of the United States of America 2024 · PMID 39365820 · doi:10.1073/pnas.2408549121
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Also filed as BioProject PRJNA1150494 and SRA study SRP527737. Searching any of these in the dataset finder brings you back here.

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