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Global Characterization of Functional Microglia Heterogeneity in Relation to Neovascularization and Regression Phases of Oxygen-Induced Retinopathy in Mice

GSE275394 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/02/08 Platform GPL24247
Summary
Retinal neovascularization (RNV) is manifested in various retinal pathological conditions, often leading to irreversible blindness. Oxygen-induced Retinopathy (OIR) mouse model proves to be useful for understanding RNV. The OIR vasculatures exhibited two distinct phases: neovascularization and regression, with microglia playing a pivotal role. Most studies concern microglia functions in promoting RNV; however, global retinal microglia dynamics and functional molecular features during RNV formation and regression have yet to be illustrated. Here, we enriched retinal microglia from normoxia (NOX) and OIR mice retinas at different timepoints of RNV progression and regression and performed single-cell RNA sequencing (scRNA-seq).This approach enabled a detailed analysis of microglia dynamics and functional heterogeneity during retinal neovascularization and regression in OIR mice, providing novel mechanistic insights with potential therapeutic implications for retinal diseases involving pathological neovascularization.
Published in
Stage-Associated Microglial Subpopulations and Dynamics in Vascular Pathogenesis of Oxygen-Induced Retinopathy
Ma Y, Chen Z, Liu B et al. · Cell proliferation 2026 · PMID 41569006 · doi:10.1111/cpr.70165
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Direct links to NCBI, no account and no request form: the whole study as GSE275394_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1150598 and SRA study SRP527872. Searching any of these in the dataset finder brings you back here.

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