← BioTransfer GEO Dataset Finder
GEO series

Tachykinin signaling defines distinct populations of glia in the enteric nervous system [Bulk RNA-seq]

GSE275794 Mus musculus (mouse) RNA-seq (gene expression) 19 samples Submitted 2026/01/28 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 19 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
19 samples: 7 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Tachykinin signaling defines distinct populations of glia in the enteric nervous systemNeuron · 2026
Summary (written by the submitters)
One of the largest populations of glia outside the brain is in the gut. These enteric glia are involved in many functions from intestinal peristalsis to immunity, yet it is unclear if there are defined types with distinct roles in homeostasis. Comparing glia from divergent microenvironments in the mouse intestine, we found that mucosal glia resembled microglia and macrophages, while muscularis glia resembled satellite glia.
Read the full summary ↓
Tacr3, encoding the receptor for neuropeptide Neurokinin B (NKB), was enriched within muscularis glia associated with neuronal soma and was undetectable in extraintestinal glia. Genetic or pharmacological manipulation of NKB-TACR3 signaling disrupted establishment of enteric glial populations during postnatal development, and dynamically modulated intestinal motor behaviors in adult mice. Collectively, we delineate spatially, transcriptionally and functionally distinct populations of enteric glia, identify one as an unanticipated target of TACR3 antagonists in clinical use, and establish this pathway as necessary for enteric glial diversification and function.
Published in
Tachykinin signaling defines distinct populations of glia in the enteric nervous system
Muppirala AN, Mitchell PE, Courtney E et al. · Neuron 2026 · PMID 41512855 · doi:10.1016/j.neuron.2025.11.030
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275794_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1153003 and SRA study SRP528971. Searching any of these in the dataset finder brings you back here.

Study design
7 conditions, mostly in triplicate
ileum muscle, male GFPpos, ×3 ileum mucosa, female GFPneg, ×3 ileum mucosa, male GFPneg, ×3 ileum mucosa, female GFPpos, ×3 ileum muscle, male GFPneg, ×2 ileum mucosa, male GFPpos, ×2 ileum muscle, female GFPpos, ×2 ileum muscle, female GFPneg, ×1

Supports a between-group comparison across 18 samples.

How we read this: 7 replicated groups read from 19 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.