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MYC-Driven Gliosis Impairs Neuron-Glia Communication in Amyotrophic Lateral Sclerosis

GSE275841 Mus musculus (mouse) RNA-seq (gene expression) 32 samples Submitted 2025/11/20 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
32 samples: 9 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
MYC-driven gliosis impairs neuron-glia communication in amyotrophic lateral sclerosisBrain : a journal of neurology · 2026
Summary (written by the submitters)
Chronic activation of glial cells leads to the dysfunction and degeneration of motor and cortical neurons in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) with an unknown mechanism. To shed light on the molecular pathogenetic processes underlying the exordium and contribution of gliosis to disease onset and progression, we used cells, mice, and patient-derived cells modeling TDP-43, SOD1, and C9orf72-linked and sporadic ALS.
Read the full summary ↓
Our data reveal a sequential disease progression, starting with enhanced glial reactivity and proliferation, and transitioning into inflammation with upregulation of pro-inflammatory genes. Using mouse genetics, we show that expression of mutant TDP-43 in astrocytes is necessary to cause gliosis and behavioral abnormalities. Mechanistically, we show that glial MYC gain-of-function drives neurodegeneration by promoting the release of astrocytic EVs that nonetheless fail to provide trophic support to surrounding neurons. Our research reveals a novel functional role for MYC in glia-to-neuron miscommunication in ALS.
Published in
MYC-driven gliosis impairs neuron-glia communication in amyotrophic lateral sclerosis
Fioretti PV, Barbieri A, Migazzi A et al. · Brain : a journal of neurology 2026 · PMID 41004427 · doi:10.1093/brain/awaf360
This dataset
Open GSE275841 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275841_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1153274 and SRA study SRP529070. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41004427) ↗
Study design
9 conditions, mostly with about 4 replicates each
Astrocytes, TDP-43_Q331K, 6Months, ×5 Oligodendrocytes, TDP-43_Q331K, 3Months, ×4 Oligodendrocytes, TDP-43_Q331K, 6Months, ×4 Astrocytes, TDP-43_Q331K, 3Months, ×4 Microglia, TDP-43_Q331K, 10Months, ×4 Microglia, TDP-43_Q331K, 3Months, ×3 Astrocytes, TDP-43_Q331K, vehicle, ×3 Astrocytes, TDP-43_Q331K, omomyc, ×3 +1 more

Supports a between-group comparison across 32 samples.

How we read this: 9 replicated groups read from 32 sample titles; they account for 32 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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