GEO series

Estrogen Receptor 1 Signaling in Hepatic Stellate Cells Designates Resistance to Liver Fibrosis

GSE275942 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 11 samples Submitted 2025/08/21 Platform GPL28330Platform GPL29480

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 11 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Homo sapiens (human)
Samples: 11 samples: 2 × mAHSC_male vs 2 × mAHSC_female vs 2 × Control vs 2 × EstradiolGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

The prevalence and severity of liver fibrosis appear higher in men than in premenopausal women, while postmenopausal women exhibit the worsened disease. However, the pathophysiological mechanism underlying such clinical observations remains incompletely understood. Here, we show that sex hormone depletion in adult female mice exaggerates the model of liver fibrosis, while estradiol replacement in castrated male mice is sufficient to mitigate the disease severity.

Read the full summary ↓

Transcriptomic analyses and immunohistochemistry then demonstrate that both human and mouse hepatic stellate cells (HSCs), the primary cell type responsible for extracellular fibrous depositions, predominantly express the estrogen receptor 1 (ESR1). Of importance, genetic deletion of ESR1 in mouse HSCs markedly promotes liver fibrosis. Moreover, chromatin immunoprecipitation followed by sequencing (ChIP-seq) and in vitro manipulations reveal that ESR1 can directly target the expression of fibrosis-related genes in HSCs. Together, this study has elucidated a critical aspect of ESR1 signaling in the sexual dimorphism of liver fibrosis.

This dataset

Open GSE275942 in finder → Find more RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE275942_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1153926 and SRA study SRP529364. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

2 × mAHSC_male vs 2 × mAHSC_female vs 2 × Control vs 2 × Estradiol

Supports a between-group comparison across 8 samples.

How we read this: 4 replicated groups read from 11 sample titles; they account for 8 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8488418 mAHSC_male1 mAHSC_male
GSM8488419 mAHSC_male2 mAHSC_male
GSM8488420 mAHSC_female1 mAHSC_female
GSM8488421 mAHSC_female2 mAHSC_female
GSM8488422 Control_1 Control
GSM8488423 Control_2 Control
GSM8488424 Estradiol_1 Estradiol
GSM8488425 Estradiol_2 Estradiol
GSM8490658 YJlab_chip_S001_hepatic
GSM8582601 Human_HSC_M
GSM8582602 Human_HSC_F

Datasets on a similar topic

GSE244843 Relaxin Attenuates the Genomic Actions and Biological Effects of Estrogens in the Myometrium by Reducing Estrogen Receptor Alpha Phosphorylation 56 samples shares estrogen, receptor
GSE341321 Vitamin B2 Sensing by the Nuclear Receptor AhR Reprograms Hepatic Metabolism [RNA-Seq] 100 samples shares receptor, hepatic
GSE341320 Vitamin B2 Sensing by the Nuclear Receptor AhR Reprograms Hepatic Metabolism [ChIP-Seq] 54 samples shares receptor, hepatic
GSE273468 Identification of Ephrin type-B receptor 4 (EphB4) as a critical mediator of tissue fibrosis 45 samples shares receptor, fibrosis
GSE251936 Circumventing Resistance within the Ewing Sarcoma Microenvironment by Anti-MCAM Chimeric Antigen Receptor Modified Ex-vivo Expanded Natural Killer Cells Combinatorial Therapy 22 samples shares resistance, receptor
GSE43988 The Estrogen receptor alpha regulated NEAT1 long non-coding RNA promotes prostate cancer progression 9 samples shares estrogen, receptor
GSE203470 COP1 Deficiency in BRAFV600E Melanomas Confers Resistance to Inhibitors of the MAPK Pathway 44 samples shares resistance
GSE243020 The loss of an orphan nuclear receptor NR2E3 augments Wnt/β-Catenin signaling via epigenetic dysregulation that promotes the Sp1-β catenin-p300 interactions in hepatocellular carcinoma. 8 samples shares receptor

Search all RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.