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EOMES safeguards TSC chromatin accessibility by directing BRG1 to TSC-associated loci [CUT&RUN]

GSE276042 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/05/19 Platform GPL19057
Summary
EOMES is an essential transcription factor (TF) for murine trophoblast stem cell (TSC) maintenance. Despite that, the details of EOMES' function at the molecular level remain obscure. Here, we carried out rapid immunoprecipitation and mass spectrometry of endogenous protein (RIME) to identify TSC-specific protein interactors on EOMES. In addition to other established TFs involved in TSC maintenance, we found that EOMES interacts with several chromatin remodeller subunits, including BRG1. By exploiting an Eomes-degron system, we acutely depleted EOMES protein in TSCs and in parallel inhibited BRG1 function with small molecule BRM014. EOMES depletion and BRG1 inhibition resulted in reduced accessibility at largely overlapping genomic regions associated with TSC-related loci. Additionally, EOMES depletion results in misregulation of genes essential for TSC maintenance and function, as well as genes encoding cytoskeletal, cell-cell interaction and matricellular components.
Published in
Eomesodermin in conjunction with the BAF complex promotes expansion and invasion of the trophectoderm lineage
Bisia AM, Xypolita ME, Bikoff EK et al. · Nature communications 2025 · PMID 40450029 · doi:10.1038/s41467-025-60417-w
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Also filed as BioProject PRJNA1154558 and SRA study SRP529595. Searching any of these in the dataset finder brings you back here.

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