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H2O2-induced astrocytic collagen triggers neuronal death in ischemic stroke [RNA-seq]

GSE276260 Mus musculus (mouse) RNA-seq (gene expression) 9 samples Submitted 2026/03/27 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 9 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 9 samples: 3 × m_astrocyte, Control, vs 3 × m_astrocyte, H202_D1, vs 3 × m_astrocyte, H202_D3,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Oxidative stress-induced astrocytic collagen biosynthesis drives glial barrier formation and neuronal death in ischemic strokeCell metabolism · 2026

Summary (written by the submitters)

Ischemic stroke is a brain injury caused by the occlusion of blood vessels, leading to reduced blood flow, excessive oxidative stress, and the cascade of events, including the formation of the glial barrier, fibrotic scar, neuronal death, and motor impairment. Despite extensive research into stroke etiology, the specific initiation trigger for neuronal death is mainly unknown, severely limiting the development of effective treatments.

Read the full summary ↓

Here, we demonstrate that excessive H2O2 generation is the initiating factor of ischemic stroke in the photo-thrombosis (PT) mouse and non-human primate (NHP) models, with H2O2-induced astrocytic type I collagen (COL1) playing a crucial role in the cascade of stroke pathology. Treatment with KDS12025, an H2O2-decomposing peroxidase enhancer, significantly alleviates these effects by reducing H2O2-induced COL1 in extremely low doses in mouse and NHP stroke models. In cultured astrocytes, H2O2 stimulates the production of astrocytic COL1 through post-transcriptional up-regulation of Col1 mRNA by decreasing microRNA29, leading to the death of neighboring neurons. Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we observe profound alterations in N-glycan profiles, particularly fucosylated N-glycans, which reverted to normal levels in both the KDS12025 treatment and astrocytic Col1 gene-silencing groups. The gene-silencing of astrocytic Col1 or Fut8, a key enzyme responsible for core fucosylation necessary for COL1 secretion, prevents neuronal death and motor impairment. Our findings reveal that H2O2-induced astrocytic COL1 production is critical to ischemic stroke. These findings propose that targeting H2O2, astrocytic COL1, and FUT8 could be effective therapeutic strategies for ischemic stroke, offering new avenues for treatment.

Published in

Oxidative stress-induced astrocytic collagen biosynthesis drives glial barrier formation and neuronal death in ischemic stroke

Lee JH, Hwang IY, Jang HJ et al. · Cell metabolism 2026 · PMID 42049021 · doi:10.1016/j.cmet.2026.04.001

This dataset

Open GSE276260 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276260_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1156178 and SRA study SRP530285. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42049021) ↗

Study design

3 × m_astrocyte, Control, vs 3 × m_astrocyte, H202_D1, vs 3 × m_astrocyte, H202_D3,

Supports a between-group comparison across 9 samples.

How we read this: 3 replicated groups read from 9 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8494242 m_astrocyte, Control, 1 m_astrocyte, Control,
GSM8494243 m_astrocyte, Control, 2 m_astrocyte, Control,
GSM8494244 m_astrocyte, Control, 3 m_astrocyte, Control,
GSM8494245 m_astrocyte, H202_D1, 1 m_astrocyte, H202_D1,
GSM8494246 m_astrocyte, H202_D1, 2 m_astrocyte, H202_D1,
GSM8494247 m_astrocyte, H202_D1, 3 m_astrocyte, H202_D1,
GSM8494248 m_astrocyte, H202_D3, 1 m_astrocyte, H202_D3,
GSM8494249 m_astrocyte, H202_D3, 2 m_astrocyte, H202_D3,
GSM8494250 m_astrocyte, H202_D3, 3 m_astrocyte, H202_D3,

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