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STimulator of INterferon Genes (STING) primed intra-articular cellular therapy improves gait and histologic outcomes in a murine osteoarthritis model

GSE276262 Mus musculus (mouse) RNA-seq (gene expression) 20 samples Submitted 2025/02/28 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 20 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
20 samples: 5 × Ctrl vs 5 × MSC vs 5 × TLR-MSC vs 5 × STING-MSCGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis modelFrontiers in bioengineering and biotechnology · 2025
Summary (written by the submitters)
We report here the histological, transcriptomic, and movement characteristics for mice who received nonactivated and activated MSC cellular therapy, post-operatively, in a surgically induced destabilization of the medial meniscus. Treatment with STING & TLR3 activated MSCs significantly improved histological, transcriptomic, and movement parameters in the mice – with TLR3-MSCs not achieving significant improvement in the movement parameters.
Read the full summary ↓
These findings can provide an approach to cellular therapies for osteoarthritis and potentially a mechanism to adjust the treatment based upon the severity/duration of degeneration. This paper further adds functional information regarding STING-MSC, TLR3-MSC, and nonactivated MSCs cellular therapy and further supports studies and literature regarding their usage in the context of regenerative therapies and alleviation of burdensome osteoarthritic symptoms.
Published in
Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis model
Plaisance C, Chow L, Impastato R et al. · Frontiers in bioengineering and biotechnology 2025 · PMID 40474870 · doi:10.3389/fbioe.2025.1525969
This dataset
Open GSE276262 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276262_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1156182 and SRA study SRP530287. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40474870) ↗
Study design
5 × Ctrl vs 5 × MSC vs 5 × TLR-MSC vs 5 × STING-MSC

Supports a between-group comparison across 20 samples.

How we read this: 4 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.