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Alternative splicing in mechanically stretched podocytes as a model of glomerular hypertension

GSE276267 Mus musculus (mouse) RNA-seq (gene expression) 15 samples Submitted 2025/05/13 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 15 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
15 samples: 6 × SVI cells, low-stretch, experiment 1, vs 3 × SVI cells, unstretched, experiment 1, vs 3 × SVI cells, high-stretch, experiment 2, vs 3 × SVI cells, unstretched, experiment 2,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Alternative Splicing in Mechanically Stretched Podocytes as a Model of Glomerular HypertensionJournal of the American Society of Nephrology : JASN · 2025
Summary (written by the submitters)
Alterations in pre-mRNA splicing play an important role in disease pathophysiology. However, the role of alternative splicing (AS) for podocytes in hypertensive nephropathy (HN) has not been investigated. Here we identifiedAS events that play a role in the development and progression of HN. For this, murine podocytes were exposed to mechanical stretch to model HN, after which proteins and mRNA were measured by proteomics, RNA-Seq, and analyzed by several AS detection tools.
Read the full summary ↓
We found two Shroom3 isoforms that showed significant changes in expression upon mechanical stretch, which was verified by qRT-PCR and in-situ hybridization. Furthermore, we observed an expression switch of two Myl6 isoforms after mechanical stretch. This switch is accompanied by a change in a C-terminally located amino acid sequence. In summary, mechanical stretch of cultured podocytes is an excellent model to simulate hypertensive nephropathy. In depth RNA-Seq analysis disclosed alternative splicing events and differential gene expression, which together with protein regulation improve the understanding of the pathophysiology of hypertension-induced nephropathy.
Published in
Alternative Splicing in Mechanically Stretched Podocytes as a Model of Glomerular Hypertension
Mattias F, Tsoy O, Hammer E et al. · Journal of the American Society of Nephrology : JASN 2025 · PMID 40418580 · doi:10.1681/ASN.0000000706
This dataset
Open GSE276267 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276267_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1156188 and SRA study SRP530297. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40418580) ↗
Study design
6 × SVI cells, low-stretch, experiment 1, vs 3 × SVI cells, unstretched, experiment 1, vs 3 × SVI cells, high-stretch, experiment 2, vs 3 × SVI cells, unstretched, experiment 2,

Supports a between-group comparison across 15 samples.

How we read this: 4 replicated groups read from 15 sample titles; they account for 15 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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