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Gene expression profiles of healthy mononuclear cells and eosinophils and asthma (moderate and severe) eosinophils

GSE276583 Homo sapiens (human) Single-cell RNA-seq 17 samples Submitted 2025/06/10 Platform GPL24676Platform GPL18573
At a glance

Single-cell RNA-seq · human · 17 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Homo sapiens (human)
Samples
17 samples: 17 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signatureiScience · 2025
Summary (written by the submitters)
We used scRNAseq to better characterize human eosinophils at resting state in healthy patients (compared to other MNCs). We then compared the healthy eosinophils to eosinophils from asthmatic patients (moderate and severe).
Published in
Single-cell RNA-sequencing of circulating eosinophils from asthma patients reveals an inflammatory signature
Goss K, Grant ML, Caldwell C et al. · iScience 2025 · PMID 40538440 · doi:10.1016/j.isci.2025.112609
This dataset
Open GSE276583 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE276583_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1157872 and SRA study SRP531210. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40538440) ↗
Study design
17 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 17 sample titles: 17 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.