RNA-sequencing of primary wild-type (WT) and Pla2g4d-deficient (KO) keratinocytes

PLA2G4D is a phospholipase and transacylase expressed in epidermal keratinocytes, and its expression is upregulated in inflammatory skin disorders such as psoriasis. Treatment of primary keratinocytes with proinflammarory cytokines IL17A and TNFα markedly increases Pla2g4d-mRNA expression. To investigate the function of PLA2G4D, we conducted bulk RNA sequencing of primary keratinocytes isolated from wild-type and Pla2g4d-deficient mice and studied transcriptional changes under basal and cytokine stimulation. The sequencing data suggests that PLA2G4D modulates fundamental biological processes including cell proliferation, differentiation, and signaling.