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Integrated multi-omics analysis of zinc finger proteins uncovers roles in RNA regulation [RNA-seq]

GSE277160 Homo sapiens (human) RNA-seq (gene expression) 242 samples Submitted 2024/09/20 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 242 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
242 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Integrated multi-omics analysis of zinc-finger proteins uncovers roles in RNA regulationMolecular cell · 2024
Summary (written by the submitters)
RNA interactome studies have revealed that hundreds of zinc finger proteins (ZFPs) are candidate RNA-binding proteins (RBPs), despite being annotated as DNA-interacting transcription factors. The RNA substrates of ZFPs and the functional significance of their potential roles as DNA- and RNA-binding proteins (DRBPs) remain largely uncharacterized.
Read the full summary ↓
Here we present a systematic multi-omics analysis of the DNA and RNA binding targets and regulatory roles of more than 100 ZFPs representing 37 zinc finger families. We show that multiple ZFPs are novel regulators of RNA splicing, polyadenylation, or stability. The examined ZFPs show widespread sequence-specific RNA binding ability and preferentially bind proximal to transcription start sites. Additionally, several ZFPs are DRBPs that bind their targets at both the DNA and RNA levels. We highlight ZNF277, a C2H2 ZFP that binds thousands of RNA targets and acts as a multi-functional RBP. We also show that ZNF473 is a DRBP that regulates the expression and splicing of cell cycle genes. Our results reveal diverse roles for ZFPs in transcriptional and post-transcriptional gene regulation, shedding light on a large class of under-studied RBPs.
Published in
Integrated multi-omics analysis of zinc-finger proteins uncovers roles in RNA regulation
Gosztyla ML, Zhan L, Olson S et al. · Molecular cell 2024 · PMID 39303722 · doi:10.1016/j.molcel.2024.08.010
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE277160_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1160885 and SRA study SRP532588. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 242 more — browse all 242 samples with per-sample file links →

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