Single-cell multiomics of peripheral blood monocytes in Crohn's Disease

Dysfunctional intestinal monocyte-derived macrophages contribute to pathology in Inflammatory Bowel Disease (IBD). Signals from the tissue micro-environment contribute to this dysfunction, however growing evidence suggests that monocytes can be primed for inflammatory function prior to tissue recruitment. Here we use single-nucleus multiomics, comprising RNA sequencing (RNA-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-seq) to identify regulatory elements underlying the altered transcriptional profile in monocytes from newly diagnosed, treatment naive Crohn’s disease patients.