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Single-cell RNA transcriptome shows IFITM1 regulates stem cell activation in limbal epithelium in response to alkali burn.

GSE277598 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/05/19 Platform GPL24247
Summary
To examine whether the upregulation of Ifitm1 is essential for the expansion of stem/early TA cells in response to corneal injury, wild type mouse eyes were topically treated with AAV-shRNA-Ifitm1 or AAV-empty vector (control) and subsequently were subjected to corneal NaOH burn. AAV-shRNA-Ifitm1 treatment downregulated Ifitm1 expression in mouse limbal epithelium . A scRNA-seq assay was conducted using cells isolated from corneal/limbal tissues at three days after corneas were injured. scRNA-seq assay identified stem/early TA cell population using Gpha2, a limbal epithelial stem /eTA cell marker. In AAV-EV treated mouse eyes, NaOH injury increased the numbers of the stem/eTA cells compared to uninjured eyes. Such expansion of stem/eTA cell population following corneal injury was markedly reduced in AAV-shRNA-Ifitm1 treated eyes. This suggests a positive role of Ifitm1 in stem/early TA cell expansion after corneal injury.
Published in
IFITM1/OVOL1 Axis Is a Novel Regulator of the Expansion of the Limbal Epithelial Stem/Early Transient Amplifying Cell Population
Jiang H, Foroozandeh P, Kaplan N et al. · FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 · PMID 40372397 · doi:10.1096/fj.202500783R
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Also filed as BioProject PRJNA1162888 and SRA study SRP533699. Searching any of these in the dataset finder brings you back here.

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