Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling
Single-nucleus RNA-seq · human · 1 samples
- What it is
- Single-nucleus RNA-seqGene activity measured in individual nuclei (used when whole cells are hard to isolate, for example from frozen tissue).
- Organism
- Homo sapiens (human)
- Samples
- 1 samples
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profilingNature biotechnology · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE277747_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1160410 and SRA study SRP533379. Searching any of these in the dataset finder brings you back here.
The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.
+ 1 more — browse all 1 samples with per-sample file links →
- GSE92872 Pooled CRISPR screening with single-cell transcriptome read-out 100 samples shares crispr, pooled
- GSE272457 Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER [scRNA-seq] 16 samples shares crispr, screens
- GSE281868 Identification of epigenetic regulators of fibrotic transformation in cardiac fibroblasts through bulk and single-cell CRISPR screens (ATAC-Seq_2) 12 samples shares crispr, screens
- GSE335904 In Vivo CRISPR Screens Identify SLC1A5 as a Metabolic Checkpoint for Cancer Immunotherapy [ATAC] 4 samples shares crispr, screens
- GSE65360 Single-cell chromatin accessibility data using scATAC-seq 1632 samples shares accessibility, chromatin
- GSE336954 Central amygdala single-nucleus atlas reveals chromatin and gene transcription dynamics in human alcohol use disorder 126 samples shares single-nucleus, chromatin
- GSE261634 Mutant p53 alters chromatin accessibility in hematopoietic stem and progenitor cells. 37 samples shares accessibility, chromatin
- GSE291155 Unlocking single cell chromatin accessibility in formalin-fixed paraffin-embedded tissues by scFFPE-ATAC 8 samples shares accessibility, chromatin
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.