Cryptic splice sites generate heterogeneous EZH2 isoforms with distinct functions in embryonic development [CUT&Tag]
ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 20 samples
- What it is
- ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
- Organism
- Mus musculus (mouse)
- Samples
- 20 samples: 8 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic developmentNucleic acids research · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE278313_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.
Also filed as BioProject PRJNA1166312 and SRA study SRP535407. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 16 samples.
How we read this: 8 replicated groups read from 20 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8544661 Ezh2a, mutation, H3K27me3, rep1 Ezh2a, mutation, H3K27m…
- GSM8544662 Ezh2a, mutation, H3K27me3, rep2 Ezh2a, mutation, H3K27m…
- GSM8544663 Ezh2a, mutation, EZH2, rep1 Ezh2a, mutation, EZH2,
- GSM8544664 Ezh2a, mutation, EZH2, rep2 Ezh2a, mutation, EZH2,
- GSM8544665 Ezh2a, mutation, IgG
- GSM8544666 Ezh2a, wild type, H3K27me3, rep1 Ezh2a, wild type, H3K27…
- GSM8544667 Ezh2a, wild type, H3K27me3, rep2 Ezh2a, wild type, H3K27…
- GSM8544668 Ezh2a, wild type, EZH2, rep1 Ezh2a, wild type, EZH2,
- GSM8544669 Ezh2a, wild type, EZH2, rep2 Ezh2a, wild type, EZH2,
- GSM8544670 Ezh2a, wild type, IgG
- GSM8544671 Ezh2b, mutation, H3K27me3, rep1 Ezh2b, mutation, H3K27m…
- GSM8544672 Ezh2b, mutation, H3K27me3, rep2 Ezh2b, mutation, H3K27m…
- GSM8544673 Ezh2b, mutation, EZH2, rep1 Ezh2b, mutation, EZH2,
- GSM8544674 Ezh2b, mutation, EZH2, rep2 Ezh2b, mutation, EZH2,
- GSM8544675 Ezh2b, mutation, IgG
- GSM8544676 Ezh2b, wild type, H3K27me3, rep1 Ezh2b, wild type, H3K27…
- GSM8544677 Ezh2b, wild type, H3K27me3, rep2 Ezh2b, wild type, H3K27…
- GSM8544678 Ezh2b, wild type, EZH2, rep1 Ezh2b, wild type, EZH2,
- GSM8544679 Ezh2b, wild type, EZH2, rep2 Ezh2b, wild type, EZH2,
- GSM8544680 Ezh2b, wild type, IgG
- GSE281926 Classes of Lamin-Associated Domains are Defined by Distinct Patterns of Repressive Histone Methylation [CUT&Tag] 18 samples shares cut, distinct
- GSE302605 Functional Comparison to Ezh2 Reveals PRC2-Independent Functions of Jarid2 in Hematopoietic Stem Cell Lineage Commitment 23 samples shares ezh2, functions
- GSE278767 Decoding the Multiple Functions of ZBP1 in the Mechanism of Sepsis-Induced Acute Lung Injury 14 samples shares cut, functions
- GSE310209 Opposing functions of AEBP2 isoforms fine-tune PRC2 catalytic activity 72 samples shares functions, isoforms
- GSE270036 Investigate the functions of core pluripotency transcription factors in embryonic stem cells 30 samples shares functions, embryonic
- GSE304211 H4K16ac contributes to chromatin compartment reorganization during mitotic and meiotic transitions [CUTnTAG] 128 samples shares cut
- GSE297309 SKP1A-mediated degradation of PRC2 triggers activation of Polycomb-silenced gene [CUT&Tag] 108 samples shares cut
- GSE303384 Mechanism of maintenance and establishment of repression by the Mtg16 tumor suppressor [CUT&RUN, ChIP-Seq] 92 samples shares cut
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.