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Increased locomotor activity does not mitigate the effects of advanced maternal age in a mouse model

GSE280473 Mus musculus (mouse) RNA-seq (gene expression) 61 samples Submitted 2025/05/10 Platform GPL30172
At a glance

RNA-seq (gene expression) · mouse · 61 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
61 samples: 20 × Placenta, Young_11_5, vs 18 × Placenta, Aged, vs 17 × Placenta, Aged_Exercise, vs 6 × Placenta, Young_10_5,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Increased locomotor activity does not mitigate the effects of advanced maternal age in a mouse modelPlacenta · 2025
Summary (written by the submitters)
Introduction: Advanced maternal age (AMA) increases the risk of pregnancy complications, in part due to impaired placentation. While exercise during pregnancy can improve outcomes, its potential to mitigate the effects of AMA has not been investigated. We evaluated the impact of exercise in a mouse model of AMA. Methods: Females were paired with males at 9 or 34 weeks of age, with one group of aged females having access to running wheels four weeks prior to and during pregnancy.
Read the full summary ↓
Pregnant females (N = 19 per group) were collected at gestational day (GD) 11.5. Placentas were collected for RNA sequencing (N = 17-20 per group). Results: Aged females without access to running wheels had heavier fat depots, while those with access to running wheels did not differ from young females. The number of viable conceptuses and fetal size were lower in both groups of aged females. Hundreds of genes were differentially expressed between young females and each of the aged groups, but only one gene was affected by access to running wheels. The placental transcriptomes of both aged groups were more similar to that of young mice at GD 10.5 than to young mice at GD 11.5, suggesting delayed placental development. Conclusions: Our model reproduced previously-reported effects of age on fetal development and placental transcriptomics, but these were not mitigated by increased voluntary locomotor activity, despite a reduction in adiposity. Remarkably, increased voluntary locomotor activity had almost no effects on placental gene expression in aged mice.
Published in
Increased locomotor activity does not mitigate the effects of advanced maternal age in a mouse model
Osorio Nieto ME, Hercus JC, Williams KR et al. · Placenta 2025 · PMID 40373689 · doi:10.1016/j.placenta.2025.05.007
This dataset
Open GSE280473 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE280473_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1178662 and SRA study SRP541599. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40373689) ↗
Study design
20 × Placenta, Young_11_5, vs 18 × Placenta, Aged, vs 17 × Placenta, Aged_Exercise, vs 6 × Placenta, Young_10_5,

Supports a between-group comparison across 61 samples.

How we read this: 4 replicated groups read from 61 sample titles; they account for 61 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 21 more — browse all 61 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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