← BioTransfer GEO Dataset Finder
GEO series

identify the secreted gene in Casp8-silenced mouse hepatocyte cell line

GSE280901 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/02/01 Platform GPL24247
Summary
Hepatocyte caspase-8 is elevated in MASH and promotes fibrosis independently of apoptosis changes. To investigate how hepatocyte caspase-8 might drive liver fibrosis progression in MASH, we focused on activated hepatic stellate cells (HSCs), which are the primary source of collagen-producing myofibroblasts and central players in MASH fibrosis. To assess a potential link between hepatocyte caspase-8 and HSC activation, we used an ex vivo model where primary murine HSCs were cultured with conditioned media from siCasp8-treated or control primary hepatocytes. Supporting our hypothesis, HSC activation markers were reduced in HSCs exposed to conditioned media from Casp8-silenced AML12 hepatocytes compared to controls. To pinpoint caspase-8-dependent secretory proteins that could activate HSCs, we conducted RNAseq on these cells, identifying secretory genes reduced in Casp8-silenced AML12 hepatocytes.
Published in
A non-apoptotic caspase-8-meteorin pathway in hepatocytes promotes MASH fibrosis
Wang X, Moore MP, Shi H et al. · Nature metabolism 2025 · PMID 41006904 · doi:10.1038/s42255-025-01355-1
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE280901_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1181012 and SRA study SRP542674. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 8 more — browse all 8 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.